The TSRF provides a variety of services to users. Investigators are requested to contact the TSRF Administrator (TSRF@ucdavis.edu) to provide information on study requirements and to complete the necessary forms for use of the facility. A meeting with scientific staff will be arranged to facilitate entry and use of the facility.

• Project consultation
• Feeders (mouse embryonic fibroblasts, MEFs)
• Pluripotent Stem Cell Training
• Media preparation and lot testing
• Cryopreservation and cell storage
• Maintaining cell banks and karyotyping
• Designing primers and probes for PCR
• DNA/RNA preparation, real-time PCR
• Quality control testing (e.g., endotoxin and mycoplasma)
• Embedding, sectioning, staining (fixed, frozen sections)
• Morphological assessments (e.g., phase contrast, brightfield, flourescence)
• Immunocytochemical analyses
• Flow cytometry, cell sorting
• Microtome
• Cryostat
• Tissue engineering strategies
• Differentiated human pluripotent stem cells for transplantation or organoid culture

Non-UC personnel subject to an additional 57% charge for established rates as per UC policy.

TSRF staff will also provide assistance with receipt of cell lines, training, and guidance in experiment setup. Individualized training can be arranged.

For a list of available equipment click here.

To download the TSRF Flyer, click here.